Real time PCR
Quantitative PCR (qPCR), also called real-time PCR or quantitative real-time PCR, is a PCR-based technique that couples amplification of a target DNA sequence with quantification of the concentration of that DNA species in the reaction.
It works on two chemistry
1. Probe based
2. Sybrgreen based chemistry.
Let's have a look at probe based principal image. Try to understand this concept.